‘Indeed, that is about appropriate. I search for a Bodily idea of biological techniques inside of a bottom-up way. I feel you could distinguish 3 strains of analysis in my team in the meanwhile. The main considerations chromosome framework: How does one obtain a metre-prolonged DNA polymer folded and organised in such a microscopic cell? We now have formulated visualisation methods that make it possible for us to check how, for instance, SMC proteins loop DNA (video clip on Youtube) so as to build up chromosomes.
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Multicolor imaging on the bacterial nucleoid and cell division proteins with blue, orange, and near-infrared fluorescent proteins
Non-equilibrium folding of unique DNA molecules recaptured nearly a thousand situations inside of a good point out nanopore
2010, designed a completely new way (‘wedging transfer’) to govern nanostructures; first report of DNA translocation by means of graphene nanopores; and understood hybrid nanopores by directed insertion of α-hemolysin into good-point out nanopores
SMC complexes can pass physical roadblocks larger than their ring dimensions, indicating a nontopological mechanism for DNA loop extrusion
We use nanofabricated microfluidic buildings to sample spatial boundaries for micro organism and biomimetic vesicles with reconstituted protein networks which assess the ceesdekker.net basic part of spatial confinement on the molecular and cellular level. Our jobs involve the subsequent:
Researching DNA loop extrusion by SMC proteins for chromosome architecture with solitary-molecule assays.
The rotors harness Electrical power from the nanoscale water and ion move that is certainly generated by a static chemical or electrochemical potential gradient during the nanopore, that happen to be set up via a salt gradient or used voltage, respectively. These artificial nanoengines self-Manage and function autonomously in physiological conditions, suggesting tips on how to setting up energy-transducing motors at nanoscale interfaces.
Resolving the phase measurement in condensin-driven DNA loop extrusion identifies ATP binding given that the stage-building method
‘The idea that There exists this kind of detail as being a starting, a giant bang, is now an interesting signpost to contemplate that there was a creator. But additional frequently, and on The premise of pure rationality, I also arrive at a Christian interpretation of reality. An atheistic worldview is really a naturalistic worldview: basically, you will discover only atoms and purely natural forces that we can measure.
‘That issues the artificial mobile, as Portion of the Dutch BaSyC initiative. Craig Venter appeared leading-down in All those times and recognized about 5 hundred genes that are minimally essential for just a working mobile. We wish to appear bottom-up: how can we shift from a handful of molecules to an entire Organic program?
2008, 1st observation of protein-coated DNA translocation by nanopores; solved the origin with the electrophoretic pressure on DNA in nanopores; uncovered a big velocity enhance of microtubules in electrical fields; identified an anomalous electro-hydrodynamic orientation of microtubules; and settled the origin of noise in carbon nanotubes in liquid
Dekker heeft fundamentele ontdekkingen gedaan op het gebied van koolstof nanobuisjes. Mede vanwege deze werkzaamheden is hij al jaren de meest geciteerde fysicus in Nederland. Tegenwoordig onderzoekt hij fulfilled enkel-molecuultechnieken de biofysica van moleculen in de biologische cel. Dekker is naast zijn wetenschappelijk werk ook bekend vanwege zijn belangstelling voor de relatie tussen geloof en wetenschap en de discussie rond clever style in 2005. (nl)